Difference between radioactive and non-radioactive probes

Radioactive and non-radioactive probes are two common probes used for nucleic acid hybridization.probe test system The main difference between them is the labeling method and nature.

Radioactive probes are single-stranded DNA or RNA sequences labeled with radioactive isotopes, while non-radioactive probes are single-stranded DNA or RNA sequences labeled using chemical or fluorescent labels. In the nucleic acid hybridization technique, nucleic acids are immobilized onto a solid surface and hybridized with a probe. If the target sequence is present in the sample, the probe will hybridize to it and make it detectable. There are two types of probes commonly used: radioactive and non-radioactive probes. Therefore, we have the option of labeling the probes with either a radioactive label or a fluorescent label.

Radioactive probes are single-stranded DNA or RNA fragments that are radioactively labeled. Radioactive isotopes are used to prepare radioactive probes. Commonly used radioisotopes include 32P, 33P, and 35S, while 3H and 125I are also less commonly used for probe labeling.RF probes Among these radioisotopes, 32P is the most commonly used isotope for labeling radioactive probes.

Radioactive probes offer a higher degree of reliability and specificity, thus providing maximum sensitivity and allowing accurate quantitative analysis of target sequences. However, radioactive probes have some drawbacks. First, they have a short half-life, which means that the marker needs to be changed periodically. Secondly, radioactive probes are hazardous and their production, use and disposal during handling are problematic. In addition, the process of preparing radioactive probes is expensive. Because of these safety and cost issues, radioactive probes are now rarely used as an alternative to non-radioactive probes.

Non-radioactive probes are the second type of probes that are labeled using chemical labels.Digoxigenin is a commonly used non-radioactive probe that is an antibody-based marker.Digoxigenin probes are specific and sensitive. Biotin is another commonly used marker for non-radioactive probe preparation.probe card Biotin/streptavidin and Digoxigenin/antibody detection systems are the most commonly used non-radioactive probe systems in hybridization. In addition, the horseradish peroxidase system is also a common non-radioactive probe system. Once these non-radioactive probes hybridize to the target sequence, they can be detected by radioautography or other imaging techniques.

Non-radioactive probes are more commonly used than radioactive probes in nucleic acid hybridization. This is because non-radioactive probes are not dangerous or radioactive. In addition, non-radioactive probes require only a shorter exposure time to detect hybridization signals. However, the steps involved in DNA hybridization with non-radioactive probes are usually tedious and time-consuming. Of course, the high price of commercial detection solutions is also a consideration.

Despite the similarities between radioactive and non-radioactive probes in nucleic acid hybridization, they have some differences. First, they differ in the way they are labeled: radioactive probes are labeled with radioisotopes, while non-radioactive probes are labeled with chemical or fluorescent labels. Secondly, they differ in their nature: radioactive probes are hazardous and have a short half-life, whereas non-radioactive probes do not have these disadvantages. Finally, there are some differences in their practical application: radioactive probes are now rarely used as an alternative to non-radioactive probes because of safety concerns and cost.

common probes peroxidase system non-radioactive probes

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